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biotinylated horse antigoat igg secondary antibody  (Vector Laboratories)


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    Vector Laboratories biotinylated horse antigoat igg secondary antibody
    Biotinylated Horse Antigoat Igg Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1677 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/horse+antigoat+igg/Biotinylated+Horse+Anti-Goat+IgG+Antibody/pmc12572975-133-1-8
    Average 96 stars, based on 1677 article reviews
    biotinylated horse antigoat igg secondary antibody - by Bioz Stars, 2026-09
    96/100 stars

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    Incubation:

    Article Title: The central amygdala projection to the substantia nigra reflects prediction error information in appetitive conditioning
    Article Snippet: The primary antibodies used were: rabbit FG antibody (1:5000–10,000 dilution; Chemicon #AB153), goat CTb antibody (1:5000–10,000 dilution; List Biological Laboratories #703), and rabbit Fos antibody (1:5000 dilution; Santa Cruz Biotechnology #sc-52). .. After the primary antibody incubation (48–72 h at 4°C), sections were rinsed in 0.1 M PB containing 0.9% saline (PBS), incubated in the appropriate biotinylated secondary antibodies such as goat antirabbit IgG or horse antigoat IgG (1:250 dilution; Vector Laboratories) for 1–1.5 h, rinsed in PBS, and then incubated in avidin–biotin peroxidase conjugate (Vector laboratories) for 1–2 h. After several rinses in PBS, sections were reacted using a Vector SG or DAB substrate kit for peroxidase (Vector Laboratories) in order to visualize Fos, FG, or CTb. ..

    Saline:

    Article Title: The central amygdala projection to the substantia nigra reflects prediction error information in appetitive conditioning
    Article Snippet: The primary antibodies used were: rabbit FG antibody (1:5000–10,000 dilution; Chemicon #AB153), goat CTb antibody (1:5000–10,000 dilution; List Biological Laboratories #703), and rabbit Fos antibody (1:5000 dilution; Santa Cruz Biotechnology #sc-52). .. After the primary antibody incubation (48–72 h at 4°C), sections were rinsed in 0.1 M PB containing 0.9% saline (PBS), incubated in the appropriate biotinylated secondary antibodies such as goat antirabbit IgG or horse antigoat IgG (1:250 dilution; Vector Laboratories) for 1–1.5 h, rinsed in PBS, and then incubated in avidin–biotin peroxidase conjugate (Vector laboratories) for 1–2 h. After several rinses in PBS, sections were reacted using a Vector SG or DAB substrate kit for peroxidase (Vector Laboratories) in order to visualize Fos, FG, or CTb. ..

    Avidin-Biotin Assay:

    Article Title: The central amygdala projection to the substantia nigra reflects prediction error information in appetitive conditioning
    Article Snippet: The primary antibodies used were: rabbit FG antibody (1:5000–10,000 dilution; Chemicon #AB153), goat CTb antibody (1:5000–10,000 dilution; List Biological Laboratories #703), and rabbit Fos antibody (1:5000 dilution; Santa Cruz Biotechnology #sc-52). .. After the primary antibody incubation (48–72 h at 4°C), sections were rinsed in 0.1 M PB containing 0.9% saline (PBS), incubated in the appropriate biotinylated secondary antibodies such as goat antirabbit IgG or horse antigoat IgG (1:250 dilution; Vector Laboratories) for 1–1.5 h, rinsed in PBS, and then incubated in avidin–biotin peroxidase conjugate (Vector laboratories) for 1–2 h. After several rinses in PBS, sections were reacted using a Vector SG or DAB substrate kit for peroxidase (Vector Laboratories) in order to visualize Fos, FG, or CTb. ..

    Article Title: Similarities and differences in the in vivo response of mouse neonatal gonocytes and spermatogonia to genotoxic stress.
    Article Snippet: The slides were then incubated in blocking buffer with either mouse monoclonal antibodies against BrdU (1:2000 in blocking buffer; Millipore) for 1 h at 378C, goat polyclonal anti-GATA 4 (1:200; Santa Cruz Biotechnology), rabbit polyclonal anti-phospho RB1 (Ser 807/811) (1:100; Cell Signaling Technology), or rabbit polyclonal anti-cleaved caspase 3 (1:100; Cell Signaling Technology) overnight at 48C. .. Primary antibodies were detected with biotinylated horse anti-mouse IgG (1:200; Vector Laboratories), horse antigoat IgG (1:200; Vector Laboratories), or goat anti-rabbit IgG (1:200; Vector Laboratories) for 30 min at room temperature and the avidin-biotin-peroxidase complex (Vectastain Elite ABC kit; Vector Laboratories). ..

    Labeling:

    Article Title: Potential Oncogenic Effect of the MERTK-Dependent Apoptotic-Cell Clearance Pathway in Starry-Sky B-Cell Lymphoma
    Article Snippet: .. Sections were labeled with monoclonal mouse antihuman CD68 clone PG-M1 (Dako M0876) or polyclonal goat antihuman MERTK (R&D AF891), and subsequently with either goat antimouse IgG, biotinylated (Vector #BA-9200) or horse antigoat IgG, biotinylated (Vector #BA-9500). .. Following incubation with Vectastain Elite ABC Reagent and DAB (Vector SK-4100), samples were counterstained in hematoxylin.

    other:

    Article Title: Dehydration-induced proteome changes in the rat hypothalamo-neurohypophyseal system.
    Article Snippet: Secondary antibodies used were as follows: antimouse IgG horseradish peroxidase-linked secondary antiserum raised in sheep (GE Healthcare), antirabbit IgG horseradish peroxidase-linked secondary antiserum raised in sheep (GE Healthcare), peroxidase-labeled horse antigoat IgG second antibody (Vector Laboratories, Burlingame, CA), biotinylated goat antirabbit or horse antigoat IgG (Vector Laboratories), streptavidin-conjugated Alexa Fluor 594 (Invitrogen), antirabbit Alexa Fluor 594, and antimouse Alexa Fluor 488 (Invitrogen).



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